αcgrp (8–37)  (Bachem)

Journal:

Article Title: CALCITONIN GENE-RELATED PEPTIDE IN THE BED NUCLEUS OF THE STRIA TERMINALIS PRODUCES AN ANXIETY-LIKE PATTERN OF BEHAVIOR AND INCREASES NEURAL ACTIVATION IN ANXIETY-RELATED STRUCTURES

doi: 10.1523/JNEUROSCI.5274-10.2011

Figure Lengend Snippet: CGRP (200, 400, 800 ng/side) infused bilaterally into the lateral BNST dose-dependently increased acoustic startle response (data are expressed as percent change compared to pre-infusion startle levels for each animal.) A) Time course of CGRP effects on startle. B) Mean (+ SEM) percent change from pre-infusion baseline to the final 60 minutes of post-infusion testing. Repeated measures ANOVA indicated a significant effect of dose, and planned comparisons revealed that both 400 and 800 ng produced significant increases compared to vehicle. (*) p < 0.05 vs. vehicle.

Article Snippet: Drugs and infusion procedure CGRP and the CRGP antagonist αCGRP 8-37 (Tocris) were prepared fresh on the day of infusion in sterile saline vehicle.

Techniques: Produced

Journal:

Article Title: CALCITONIN GENE-RELATED PEPTIDE IN THE BED NUCLEUS OF THE STRIA TERMINALIS PRODUCES AN ANXIETY-LIKE PATTERN OF BEHAVIOR AND INCREASES NEURAL ACTIVATION IN ANXIETY-RELATED STRUCTURES

doi: 10.1523/JNEUROSCI.5274-10.2011

Figure Lengend Snippet: Pretreatment with the CGRP antagonist, αCGRP8-37 (400 ng/side) significantly attenuated CGRP-enhanced startle (both infused directly into the BNST), but did not affect startle amplitude when administered prior to vehicle infusion. Values shown are mean (± SEM) percent change from the pre- to post-infusion test. (†) p < 0.05 vs vehicle/vehicle. (*) p < 0.05 vs vehicle/CGRP.

Article Snippet: Drugs and infusion procedure CGRP and the CRGP antagonist αCGRP 8-37 (Tocris) were prepared fresh on the day of infusion in sterile saline vehicle.

Techniques:

Journal:

Article Title: CALCITONIN GENE-RELATED PEPTIDE IN THE BED NUCLEUS OF THE STRIA TERMINALIS PRODUCES AN ANXIETY-LIKE PATTERN OF BEHAVIOR AND INCREASES NEURAL ACTIVATION IN ANXIETY-RELATED STRUCTURES

doi: 10.1523/JNEUROSCI.5274-10.2011

Figure Lengend Snippet: CGRP (800 ng/side) infused into but not dorsal to the BNST significantly increased startle amplitude. Intra-BNST infusions of the antagonist, αCGRP8-37 did not significantly affect startle amplitude. Values shown are mean (± SEM) percent change from the pre- to post-infusion test. * p < 0.05 vs vehicle.

Article Snippet: Drugs and infusion procedure CGRP and the CRGP antagonist αCGRP 8-37 (Tocris) were prepared fresh on the day of infusion in sterile saline vehicle.

Techniques:

Journal:

Article Title: CALCITONIN GENE-RELATED PEPTIDE IN THE BED NUCLEUS OF THE STRIA TERMINALIS PRODUCES AN ANXIETY-LIKE PATTERN OF BEHAVIOR AND INCREASES NEURAL ACTIVATION IN ANXIETY-RELATED STRUCTURES

doi: 10.1523/JNEUROSCI.5274-10.2011

Figure Lengend Snippet: ICV CGRP (1.6 μg) significantly increased acoustic startle compared to vehicle Values shown are mean (± SEM) percent change from the pre- to post-infusion test.

Article Snippet: Drugs and infusion procedure CGRP and the CRGP antagonist αCGRP 8-37 (Tocris) were prepared fresh on the day of infusion in sterile saline vehicle.

Techniques:

Journal:

Article Title: CALCITONIN GENE-RELATED PEPTIDE IN THE BED NUCLEUS OF THE STRIA TERMINALIS PRODUCES AN ANXIETY-LIKE PATTERN OF BEHAVIOR AND INCREASES NEURAL ACTIVATION IN ANXIETY-RELATED STRUCTURES

doi: 10.1523/JNEUROSCI.5274-10.2011

Figure Lengend Snippet: Effect of intra-BNST CGRP (800 ng bilaterally) on c-Fos expression in anxiety, fear, and startle-related neural regions. Values are expressed as mean (± SEM) number of c-Fos-positive cells/mm 2 .

Article Snippet: Drugs and infusion procedure CGRP and the CRGP antagonist αCGRP 8-37 (Tocris) were prepared fresh on the day of infusion in sterile saline vehicle.

Techniques: Expressing, Control

Journal:

Article Title: CALCITONIN GENE-RELATED PEPTIDE IN THE BED NUCLEUS OF THE STRIA TERMINALIS PRODUCES AN ANXIETY-LIKE PATTERN OF BEHAVIOR AND INCREASES NEURAL ACTIVATION IN ANXIETY-RELATED STRUCTURES

doi: 10.1523/JNEUROSCI.5274-10.2011

Figure Lengend Snippet: Representative low-magnification brightfield photomicrographs for regions showing significant differences in c-Fos immunoreactivity density between treatment groups. Vehicle—left panels; 800 ng bilateral intra-BNST CGRP—right panels. Scale bar = 200 μm. 3v, third ventricle; 4v, fourth ventricle; ac, anterior commissure; CeA, central nucleus of the amygdala; DRN, dorsal raphe nucleus; LC, locus coeruleus; LH, lateral hypothalamus, LPB, lateral parabrachial nucleus; scp, superior cerebellar peduncle; LSv, ventral lateral septal nucleus; LSi, intermediate lateral septal nucleus; lv, lateral ventricle; NAcC, nucleus accumbens core; NAcSh, nucleus accumbens shell; PAGvl, ventrolateral periaqueductal gray; PVN, hypothalamic paraventricular nucleus.

Article Snippet: Drugs and infusion procedure CGRP and the CRGP antagonist αCGRP 8-37 (Tocris) were prepared fresh on the day of infusion in sterile saline vehicle.

Techniques:

Journal:

Article Title: CALCITONIN GENE-RELATED PEPTIDE IN THE BED NUCLEUS OF THE STRIA TERMINALIS PRODUCES AN ANXIETY-LIKE PATTERN OF BEHAVIOR AND INCREASES NEURAL ACTIVATION IN ANXIETY-RELATED STRUCTURES

doi: 10.1523/JNEUROSCI.5274-10.2011

Figure Lengend Snippet: Bilateral intra-BNST CGRP (800 ng/side) infusions produced anxiety-like behavior in the elevated plus maze. The CGRP antagonist, αCGRP8-37 (800 ng/side), had no effect. A) Percent of total time spent on the open arms. B) Percent of total entries that were made into open arms. C) Total entries. Values shown are means ± SEM. (*) p < 0.05 vs vehicle.

Article Snippet: Drugs and infusion procedure CGRP and the CRGP antagonist αCGRP 8-37 (Tocris) were prepared fresh on the day of infusion in sterile saline vehicle.

Techniques: Produced

Journal:

Article Title: A comparison of the actions of BIBN4096BS and CGRP 8–37 on CGRP and adrenomedullin receptors expressed on SK-N-MC, L6, Col 29 and Rat 2 cells

doi: 10.1038/sj.bjp.0704844

Figure Lengend Snippet: Effects of BIBN4096BS on the stimulation of cAMP production by human αCGRP in the presence of the indicated concentrations of BIBN4096BS in (a) L6 cells, (b) SK-N-MC cells and (c) Col 29 cells. Data represent means±s.e.mean of two to five experiments. Points were measured in duplicate in each experiment. Data are expressed as percentage of maximum cAMP production, estimated by fitting each line to a logistic Hill equation as described in the Methods. Maximum cAMP values were as follows: L6 cells, 270±30 pmol per 106 cells; SK-N-MC cells, 240±20 pmol per 106 cells; Col 29 cells, 170±19 pmol per 106 cells. Basal values were all below 10 pmol per 106 cells.

Article Snippet: Human αCGRP 8–37 and peptidase inhibitors were purchased from Calbiochem.

Techniques:

Journal:

Article Title: A comparison of the actions of BIBN4096BS and CGRP 8–37 on CGRP and adrenomedullin receptors expressed on SK-N-MC, L6, Col 29 and Rat 2 cells

doi: 10.1038/sj.bjp.0704844

Figure Lengend Snippet: Effects of CGRP8–37 on the stimulation of cAMP production by human αCGRP in the presence of the indicated concentrations of antagonist in (a) L6 cells, (b) SK-N-MC cells and (c) Col 29 cells. Data represent means±s.e.mean of four to seven experiments. Points were measured in duplicate in each experiment. Data are expressed as percentage of maximum cAMP production, estimated by fitting each line to a logistic Hill equation as described in the Methods. Maximum cAMP values were as follows: L6 cells, 250±27 pmol per 106 cells; SK-N-MC cells, 230±18 pmol per 106 cells; Col 29 cells, 180±17 pmol per 106 cells. Basal values were all below 10 pmol per 106 cells.

Article Snippet: Human αCGRP 8–37 and peptidase inhibitors were purchased from Calbiochem.

Techniques:

Journal:

Article Title: CGRP receptors mediating CGRP-, adrenomedullin- and amylin-induced relaxation in porcine coronary arteries. Characterization with 'Compound 1' (WO98/11128), a non-peptide antagonist

doi: 10.1038/sj.bjp.0704210

Figure Lengend Snippet: αCGRP, βCGRP, AM and amylin concentration-reponse relationship in porcine coronary arteries. Points represent mean values and vertical lines indicate s.e.mean. Relative responses are given as percentage fraction of the initial vessel response to U46619 (10−7 M) just before they were challenged with the relaxant peptides.

Article Snippet: Drugs Human αCGRP, βCGRP, AM, amylin and the fragments αCGRP 8–37 , βCGRP 8–37 and AM 22–52 were all obtained from Bachem AG, Switzerland.

Techniques: Concentration Assay

Journal:

Article Title: CGRP receptors mediating CGRP-, adrenomedullin- and amylin-induced relaxation in porcine coronary arteries. Characterization with 'Compound 1' (WO98/11128), a non-peptide antagonist

doi: 10.1038/sj.bjp.0704210

Figure Lengend Snippet: (a–d) Vasorelaxant effect of αCGRP (10−10–10−7 M) and βCGRP (10−10–10−7 M) in the porcine LAD contracted with U46619 (10−7 M). The antagonists αCGRP8–37 (10−7–10−5 M), βCGRP8–37 (10−7–10−5 M) were added 15 min and U46619 10 min prior to the αCGRP/βCGRP challenge. Points represent mean values and vertical lines indicate s.e.mean. Relative responses are given as percentage fraction of the initial vessel response to U46619 (10−7 M) just before they were challenged with αCGRP/βCGRP.

Article Snippet: Drugs Human αCGRP, βCGRP, AM, amylin and the fragments αCGRP 8–37 , βCGRP 8–37 and AM 22–52 were all obtained from Bachem AG, Switzerland.

Techniques:

Journal:

Article Title: CGRP receptors mediating CGRP-, adrenomedullin- and amylin-induced relaxation in porcine coronary arteries. Characterization with 'Compound 1' (WO98/11128), a non-peptide antagonist

doi: 10.1038/sj.bjp.0704210

Figure Lengend Snippet: Schild plots for αCGRP8–37 and βCGRP8–37 tested with human αCGRP and βCGRP as agonists in isolated porcine LAD. The Schild plot curve for αCGRP8–37 (10−7–10−5 M) tested with αCGRP was equal to 1.48× +10.3 (r=0.62; P=0.0011). The pA2 value=7.0 (6.4–8.6). The Schild plot curve for αCGRP8–37 (10−7–10−5 M) tested with βCGRP was equal to 1.23× +7.8 (r=0.63; P=0.0007). The pA2 value=6.9 (6.2–8.7). The Schild plot curve for βCGRP8–37 (10−7–10−5 M) tested with αCGRP was equal to 1.05× +7.2 (r=0.61; P=0.0031). The pA2 value=6.3 (5.9–7.0). The Schild plot curve for βCGRP8–37 (10−6–10−5 M) tested with βCGRP was equal to 1.68× +10.0 (r=0.65; P=0.0002). The pA2 value=5.9 (5.7–6.5). Each point represents mean values and vertical lines indicate s.e.mean.

Article Snippet: Drugs Human αCGRP, βCGRP, AM, amylin and the fragments αCGRP 8–37 , βCGRP 8–37 and AM 22–52 were all obtained from Bachem AG, Switzerland.

Techniques: Isolation

Journal:

Article Title: CGRP receptors mediating CGRP-, adrenomedullin- and amylin-induced relaxation in porcine coronary arteries. Characterization with 'Compound 1' (WO98/11128), a non-peptide antagonist

doi: 10.1038/sj.bjp.0704210

Figure Lengend Snippet: Vasorelaxant effect of AM in the porcine LAD contracted with U46619 (10−7 M). The proposed antagonists αCGRP8–37 (10−6 M), βCGRP8–37 (10−6 M) and AM22–52 (10−6 M) were added 15 min and U46619 10 min prior to the αCGRP (10−9–10−6 M) challenge. Points represent mean values and vertical lines indicate s.e.mean. Relative responses are given as percentage fraction of the initial vessel response to U46619 (10−7 M) just before they were challenged with AM.

Article Snippet: Drugs Human αCGRP, βCGRP, AM, amylin and the fragments αCGRP 8–37 , βCGRP 8–37 and AM 22–52 were all obtained from Bachem AG, Switzerland.

Techniques:

Journal:

Article Title: CGRP receptors mediating CGRP-, adrenomedullin- and amylin-induced relaxation in porcine coronary arteries. Characterization with 'Compound 1' (WO98/11128), a non-peptide antagonist

doi: 10.1038/sj.bjp.0704210

Figure Lengend Snippet: Vasorelaxant effect of amylin in the porcine LAD contracted with U46619 (10−7 M). The proposed antagonists αCGRP8–37 (10−6 M), βCGRP8–37 (10−6 M) and AM22–52 (10−6 M) were added 15 min and U46619 10 min prior to the αCGRP (10−9–10−6 M) challenge. Points represent mean values and vertical lines indicate s.e.mean. Relative responses are given as percentage fraction of the initial vessel response to U46619 (10−7 M) just before they were challenged with amylin.

Article Snippet: Drugs Human αCGRP, βCGRP, AM, amylin and the fragments αCGRP 8–37 , βCGRP 8–37 and AM 22–52 were all obtained from Bachem AG, Switzerland.

Techniques:

Journal:

Article Title: CGRP receptors mediating CGRP-, adrenomedullin- and amylin-induced relaxation in porcine coronary arteries. Characterization with 'Compound 1' (WO98/11128), a non-peptide antagonist

doi: 10.1038/sj.bjp.0704210

Figure Lengend Snippet: (a,b) Vasorelaxant effect of αCGRP (10−10–10−7 M) and βCGRP (10−10–10−7 M) in the porcine LAD contracted with U46619 (10−7 M). The antagonist AM22–52 (10−6 M) was added 15 min and U46619 10 min prior to the αCGRP/βCGRP challenge. Points represent mean values and vertical lines indicate s.e.mean. Relative responses are given as percentage fraction of the initial vessel response to U46619 (10−7 M) just before they were challenged with αCGRP/βCGRP.

Article Snippet: Drugs Human αCGRP, βCGRP, AM, amylin and the fragments αCGRP 8–37 , βCGRP 8–37 and AM 22–52 were all obtained from Bachem AG, Switzerland.

Techniques:

Journal:

Article Title: CGRP receptors mediating CGRP-, adrenomedullin- and amylin-induced relaxation in porcine coronary arteries. Characterization with 'Compound 1' (WO98/11128), a non-peptide antagonist

doi: 10.1038/sj.bjp.0704210

Figure Lengend Snippet: Vasorelaxant effect of αCGRP in the porcine LAD contracted with U46619 (10−7 M). The non-peptide CGRP antagonist ‘Compound 1' (10−7–10−5 M) was added 15 min and U46619 10 min prior to the αCGRP (10−10–10−7 M) challenge. Points represent mean values and vertical lines indicate s.e.mean. Relative responses are given as percentage fraction of the initial vessel response to U46619 (10−7 M) just before they were challenged with αCGRP.

Article Snippet: Drugs Human αCGRP, βCGRP, AM, amylin and the fragments αCGRP 8–37 , βCGRP 8–37 and AM 22–52 were all obtained from Bachem AG, Switzerland.

Techniques: